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Part:BBa_K1093024:Design

Designed by: Jakub Piątkowski   Group: iGEM13_Warsaw   (2013-09-23)


MinC/Lysis device-based kill switch with delayed expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 4693
    Illegal NheI site found at 4716
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2006
    Illegal AgeI site found at 4298
    Illegal AgeI site found at 4368
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3278
    Illegal SapI site found at 4949


Design Notes

Delay is obtained with the use of LuxI/LuxR system


Source

The sequence was synthetized by GeneRay

References